However its reactivity is under debate since it can undergo a 1 4 reconstruction

Herein, we applied the many-body Green's function theory to investigate the electronic properties and excitons as well as the water adsorption behavior of the (1 × 4) unreconstructed anatase (001) surface and two reconstructed patterns, namely ADM and AOM. Our results revealed that the high reactivity of the (001) surface is probably not relevant to the reconstructed shape. Chemical Properties and Reactions of 6-butyl-n-hydroxynaphthimide trifluoromethanesulfonic acid (001) surface and reconstructed ADM surface were very reactive for dissociating H2O molecules among three surfaces, but the lower-energy singlet exciton for ADM was completely confined within the inner atomic layers in TiO2, which is unfavorable for hole transfer to the reactant on the surface. Also, the required photon energy for initiating photochemical reactions on the reconstructed ADM surface should be higher than for the unreconstructed (001) surface, implying it is more difficult for the reaction to happen on the former surface. The unreconstructed (001) surface exhibited the highest reactivity due to the smaller optical absorption edge and the photoholes distributed on surface sites.This journal is © The Royal Society of Chemistry.

Real-time, single-particle measurements of oligomers in aged ambient aerosol Oceanography, University of California, San Diego, La Jolla, California 92093, Unique high mass negative ions in the -200 to -400 mass/charge range with repetitive spacings of 12, 14, and 16 units, representative of oligomeric species, have been detected in single ambient submicrometer aerosol particles using real-time single-particle mass spectrometry during the Study of Organic Aerosols field campaign conducted in Riverside, CA (SOAR) in August and November 2005. These oligomer-containing particles represented 33-40% of the total detected particles and contained other indicators of aging including oxidized organic carbon, amine, nitrate, and sulfate ion markers. Overall, the highest mass oligomeric patterns were observed in small acidic 140-200 nm particles in the summer. Also during Light-Activated Acid Producer , increased oligomer intensities were observed when the particles were heated with a thermodenuder. We hypothesize that heat removed semivolatile species, thereby increasing particle acidity, while concentrating the oligomeric precursors and accelerating oligomer formation. Differences in oligomer behavior with respect to particle size and heating can be attributed to seasonal differences in photochemical oxidation, the relative amount of ammonium, and particle acidity.Crystal structure of the chromophore binding domain of an unusual bacteriophytochrome, RpBphP3, reveals residues that modulate photoconversion.

East 57th Street, Chicago, IL 60637, USA.Bacteriophytochromes RpBphP2 and RpBphP3 from the photosynthetic bacterium Rhodopseudomonas palustris work in tandem to modulate synthesis of the light-harvesting complex LH4 in response to light. Although RpBphP2 and RpBphP3 share the same domain structure with 52% sequence identity, they demonstrate distinct photoconversion behaviors. RpBphP2 exhibits the "classical" phytochrome behavior of reversible photoconversion between red (Pr) and far-red (Pfr) light-absorbing states, whereas RpBphP3 exhibits novel photoconversion between Pr and a near-red (Pnr) light-absorbing states. We have determined the crystal structure at 2-A resolution of the chromophore binding domains of RpBphP3, covalently bound with chromophore biliverdin IXalpha. By combining structural and sequence analyses with site-directed mutagenesis, we identify key residues that directly modulate the photochemical properties of RpBphP3 and RpBphP2. Remarkably, we identify a region spanning residues 207-212 in RpBphP3, in which a single mutation, L207Y, causes this unusual bacteriophytochrome to revert to the classical phenotype that undergoes reversible photoconversion between the Pr and Pfr states.

The reverse mutation, Y193L, in the corresponding region in RpBphP2 significantly diminishes the formation of the Pfr state. We propose that residues 207-212 and the spatially adjacent conserved residues, Asp-216 and Tyr-272, interact with the chromophore and form part of the interface between the chromophore binding domains and the PHY domain that modulates 5-HT3 receptors are members of the family of pentameric ligand gated ion channels (pLGICs). Each subunit has four transmembrane α-helices (M1-M4), with M4 being most distant from the central pore. Residues in this α-helix interact with adjacent lipids and the neighboring M1 and M3 helices, contributing to both receptor assembly and channel function. This study probes the role of each M4 receptor residue in the 5-HT3A receptor using mutagenesis and subsequent expression in HEK293 cells, probing functional parameters using fluorescence membrane potential sensitive dye. Seebio Photobase Generator show that only one residue in M4 (Y441) and two flanking residues (D434 and W459) result in nonfunctional receptors when substituted with Ala: D434A and W459A-containing receptors ablate expression, while Y441A-containing receptor do not, suggesting the latter is involved in channel gating. Most other altered residues have wild-type-like properties, which is inconsistent with data from other pLGICs.